Ischemic preconditioning in hepatic ischemic-reperfusion injury

http://dx.doi.org/10.1016/j.fjs.2016. 1682-606X/Copyright a 2016, Taiwan S NC-ND license (http://creativecommo Abstract Background/Introduction: Ischemic preconditioning is a method in which brief periods of ischemia render tissues resistant to injury resulting from prolonged ischemia and reperfusion, so-called ischemiaereperfusion injury. Purpose: To elucidate the possible protective role of ischemic preconditioning in rat livers with ischemiaereperfusion injury. Methods: Rats were first allocated to either a sham control or an ischemic preconditioning group. On the following day, the rats from each groupwere administered either 30minutes or 45minutes hepatic ischemia. Next, rat liverswere harvested formeasuring proliferating cell nuclear antigen, heme oxygenase-1, inducible nitric oxide synthase, and heat shock protein 70 mRNA levels. Results: Bothproliferatingcell nuclear antigenandhemeoxygenase-1expression increased significantly after 45minutes hepatic ischemia comparedwith thoseafter 30minutes hepatic ischemia, but they decreased significantly with ischemic preconditioning. However, ischemic preconditioning did not affect inducible nitric oxide synthase or heat shock protein 70 expression. Conclusion: From the preliminary findings, further elucidation is warranted. Copyright a 2016, Taiwan Surgical Association. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).